A new way to measure the age of dolphins opens a window onto the lives of these iconic animals

Knowing the age of an animal reveals key information about how long it lives and when it reproduces.

Age is also essential to understanding the health of a population and how well it can cope with threats such as pollution and habitat loss.

But figuring out the age of wild animals is not easy, especially for dolphins.

Until recently, researchers had to slice the teeth of dead dolphins and count growth layers, much like tree rings.

This method becomes more problematic in older animals because teeth wear and the growth layers sit closer together, which can lead to dolphins’ age being underestimated. It is obviously also impossible to extract teeth from living dolphins.

In our new research, we linked DNA markers with known tooth ages of dead dolphins to build the first molecular “epigenetic clock” for common dolphins (Delphinus delphis).

This allows scientists to estimate the age of living common dolphins from just a skin sample, opening a new window onto the lives of these iconic animals.

But this research also raises two key questions: could the limitations of tooth-based aging affect the accuracy of the clock, and does DNA degradation in dead animals influence the age estimates? Our work addresses both concerns.

How DNA becomes a clock

New DNA-based techniques are transforming how scientists estimate age by reading tiny chemical changes in DNA that change predictably as animals grow older.

Researchers have studied these DNA patterns in hundreds of mammal species and developed epigenetic clocks—reliable tools that estimate age without having to remove teeth or use invasive methods.

While epigenetic clocks are now emerging for dolphins, these DNA changes have only been studied in a few species so far. Until now, no epigenetic clock has existed for common dolphins—one of the world’s most widespread dolphin species.

This is because scientists first need skin samples from animals whose ages are already known to build these clocks.

These reference ages often come from dolphins monitored since birth in the wild or from animals in human care. But for many long-lived species, such long-term records simply don’t exist.

A handful of studies used tooth ages from dead dolphins, but this has historically raised questions about the effect of decomposition, and also whether errors in tooth aging older animals may affect accuracy of the DNA clocks.

To address these concerns, we analyzed 75 common dolphins that had stranded or been accidentally caught in fishing nets in New Zealand. For each animal, we counted growth layers in teeth to determine age and used a skin sample to measure DNA markers at almost 38,000 sites across the genome.

We combined these data to build a model that estimates age from DNA to create an epigenetic clock for common dolphins.

Putting the clock to the test

Our clock can predict age to within about two years, although estimates became less precise in older dolphins.

This brings us back to our key question: is this due to less accurate tooth aging in older animals, or because DNA breaks down after death?

In our study, the clock tended to underestimate the age of older dolphins. However, if tooth aging were the problem, we would expect the opposite—that the DNA clock would give older ages than tooth readings.

This makes dental aging errors unlikely. We also tested whether skin decomposition affected age estimates, and found no effect.

Why then are older dolphins underestimated? This is a biological effect seen in many species. The DNA changes that track age become more gradual later in life, which means the clock has fewer signals to work with.

It’s not caused by teeth or decay, but because aging slows naturally at the molecular level.

A game changer for dolphin conservation

Our findings address longstanding concerns about whether tooth ages are reliable to calibrate epigenetic clocks. We show the method is genuinely robust, even when using stranded or by-caught dolphins.

This means DNA age clocks can be built for animals whose ages are only known from tooth readings. This is important because for many dolphin species, tooth records from dead animals are the main or only method scientists have to determine an individual’s age.

This molecular clock is a major step forward for conserving common dolphins.

Scientists can now determine age from minimally invasive biopsy samples and, in turn, estimate survival and reproductive rates in free-ranging wild dolphins.

This will help identify how threats such as climate change and fisheries’ bycatch affect different age groups.

Common dolphins are one of the most widespread dolphin species on Earth and have long been considered abundant. But they face significant human pressures globally and are increasingly vulnerable.

In New Zealand and across Australasia, they are among the most frequently caught dolphins in fisheries’ bycatch. Experience from other regions such as the Mediterranean Sea shows that even “common” species can decline rapidly under sustained pressures such as overfishing, pollution and habitat degradation.

Being able to accurately estimate age from a small skin sample means scientists can better understand how long common dolphins live, when they reproduce and how many young survive. Such information is critical for protecting populations before they decline.

This article is republished from The Conversation under a Creative Commons license. Read the original article.